|
KMID : 0613820070170030356
|
|
Journal of Life Science
2007 Volume.17 No. 3 p.356 ~ p.361
|
|
|
ErbB2 kinase domain is required for ErbB2 association with β-catenin
|
|
Jung Yun-Jin
Ha Nam-Chul
Xu Wanping
Neckers Len
|
|
|
Abstract
|
|
|
|
To investigate the region of ErbB2 for the ErbB2-β-catenin interaction, a proteasome resistant-β-catenin and various ErbB2 constructs were transfected in COS7 cells. ErbB2 proteins were immunoprecipitated, and coimmunoprecipitated β-catenin was examined by Western blotting. β-catenin coimm unoprecipitated with full length ErbB2. Of the truncated ErbB2 proteins DT (1-1123), DHC (1-1031) and DK (1-750), the ErbB2 constructs containing the kinase domain, DT and DHC, precipitated together with β-catenin but DK containing no kinase domain did not. To further test the requirement of the kinase domain for β-catenin-ErbB2 interaction, the presence of β-catenin in the immunocomplex was examined following transfection with an ErbB2 mutant (Δ750-971) whose kinase domain is internally deleted and subsequent immunoprecipitation of the ErbB2 mutant. β-catenin was not detected in the immunocomplex. These results suggest that the ErbB2 kinase domain comprises a potential site for β-catenin binding to the receptor tyrosine kinase.
|
|
|
KEYWORD
|
|
|
β-catenin, ErbB2, protein association
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|